Part:BBa_K4179001
Pastinaca sativa prenyl transferase 1 (PsPT1)
P. sativa prenyltransferase 1 (PsPT1) was discovered and characterized as a plastidic prenyltransferase, that synthesizes demethylsuberosin (DMS), a linear furanocoumarin (FC) intermediate, and osthenol an angular FC intermediate, respectively, in parsnip [1].
Prenylation
Prenylation is a structural modification in which an isoprenoid moiety, prenyl being the most common, is transferred from a donor molecule to an acceptor. Prenylation of aromatic substrates causes the enhancement of their bioactivity which is a crucial step in the biosynthesis of biologically active secondary metabolites, which are important in the survival and disease resistance of many plant species. To perform prenylation, plants use membrane-bound aromatic prenyltransferases (PTs) that transfer isoprenoid moieties from pyrophosphate donor substrates to aromatic acceptor substrates [2].
Usage and Biology
PsPT1 is an umbelliferone dimethylallyltransferase (UDT), a prenyltransferase that is specific for the first reaction step in the FC biosynthetic pathway. It catalyzes a prenylation reaction with umbelliferone as the acceptor molecule and dimethylallylpyrophosphate (DMAPP) as the donor of the prenyl group, producing DMS if the prenylation took place at the 6th carbon position of umbelliferone, and osthenol if prenylation occurred at the 8th carbon position (figure 2).
The team of Technion 2022 used this part in a construct (BBa_K4179003) designed for the purpose of introducing decursin’s biosynthetic pathway into E. coli. The team’s starting point was umbelliferone, which had to be prenylated to yield DMS. and thus, this enzyme was needed to perform the first reaction in the pathway.
Sequence and features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562
Illegal PstI site found at 55 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562
Illegal NheI site found at 1210
Illegal PstI site found at 55 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562
Illegal PstI site found at 55 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 127
Illegal EcoRI site found at 562
Illegal PstI site found at 55 - 1000COMPATIBLE WITH RFC[1000]
This part includes the gene of PsPT1, in addition to two restriction sites, one at every end of the gene. NdeI restriction site was added to the N-terminus side of the sequence, directly before the methionine codon of the PsPT1 gene. NheI restriction site was added to the C-terminus side of the sequence, directly after the end of the gene.
A stop codon is not present at the end of the PsPT1 gene, due to the gene being cloned upstream to a P2A (BBa_K4179005)-mCherry(BBa_K106005)sequence. In this genetic system (BBa_K4179003) , the mCherry expression is tied directly to the start codon of PsPT1, while not being covalently bound to it. In such a design the mCherry signal corresponds to a 1:1 ratio, at the very least, which allows for a lower estimate of PsPT1’s expression.
References
1. Munakata R, Olry A, Karamat F et al. Molecular evolution of parsnip (Pastinaca sativa) membrane-bound prenyltransferases for linear and/or angular furanocoumarin biosynthesis. New Phytologist 2016; 211: 332–344.
2. de Bruijn WJC, Levisson M, Beekwilder J, van Berkel WJH, Vincken J-P. Plant Aromatic Prenyltransferases: Tools for Microbial Cell Factories. 2020;
3. Bateman A, Martin MJ, Orchard S et al. UniProt: the universal protein knowledgebase in 2021. Nucleic Acids Res 2021; 49: D480–D489.
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